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Structure of the Ubiquitin-interacting Motif of S5a Bound to the ...
Although the N-terminal UIM of human S5a binds to polyubiquitin chains much more weakly than ... substantial changes upon binding to S5a (18 and 30). For ...
www.jbc.org/cgi/reprint/279/6/4760.pdf
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The Ubiquitin-interacting Motif Protein, S5a, Is Ubiquitinated by ...
Ubiquitination AssaysTo assay the ubiquitination of S5a, 400 nm His10-S5a was incubated ... (125 μm × 18 cm) column packed with C18 reverse-phase material ( Magic C18AQ, ..... such as t...
www.jbc.org/content/284/19/12622.full
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DNA-repair protein hHR23a alters its protein structure upon ...
One of these subunits, S5a, also recognizes polyubiquitin chains (6, 7) via ... this 80-residue domain is at the N terminus and separated from the rest of the ...
www.ncbi.nlm.nih.gov/pmc/articles/PMC240680/?tool=...
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Purification of poly-ubiquitinated proteins by S5a-affinity ...
its N-terminus, through which it interacts with S5a (Hiyama, H., et al., J Biol. Chem. ..... [18], since the denatured molecular mass of the smallest ...
dx.doi.org/10.1002/1615-9861(200106)1:6%3C773::AID...
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The N terminus of the serpin, tengpin, functions to trap the ...
... at the top of strands s3A and s5A allows the insertion of two residues of the RCL (Fig 1A) ... 1997 May 6; 36(18):5455-64. [Biochemistry. 1997] The serpin ...
www.ncbi.nlm.nih.gov/pmc/articles/PMC1905895/?tool...
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Journal of Molecular Biology : Defining how Ubiquitin Receptors ...
Therefore, this N-terminal domain is expected to mediate S5a/Rpn10 ... domains that bind the proteasome.12,13,17,18 They have attracted much attention for ...
linkinghub.elsevier.com/retrieve/pii/S002228360700...
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Rapid Recruitment of BRCA1 to DNA Double-Strand Breaks Is ...
Two small regions in the N terminus of BRCA1 independently accumulated at DSBs ... 18. Gatei, M., B. B. Zhou, K. Hobson, S. Scott, D. Young, and K. K. Khanna. 2001. ...
mcb.asm.org/cgi/content/full/28/24/7380
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Yeast counterparts of subunits S5a and p58 (S3) of the human 26S ...
Furthermore, expression of a derivative of p58 from which the N-terminal 150 amino acids had .... Genes & Dev., September 15, 2000; 14(18): 2344 - 2357. ...
www.molbiolcell.org/cgi/content/abstract/8/1/171
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Id1 Overexpression Induces Tetraploidization and Multiple ...
... N and HLH domains are essential for binding to the S5A (Hasskarl ... D box and S5A binding to the Id1 N terminus and the HLH domain. ... Genet 18, 161172. ...
www.molbiolcell.org/cgi/content/full/19/6/2389
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Deletion of proteasomal subunit S5a/Rpn10/p54 causes lethality ...
2-3 transposase source on the second chromosome and the 0554/18 P(w+) insertion ..... The discovery that the DNA repair protein Rad 23 carries an N-terminal ...
jcs.biologists.org/cgi/content/full/116/6/1023
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FAQs For IMPACT Protein Purification System, NEB
When using the N- and C-terminal fusion construct (pTWIN system) to generate ... protein without any vector-derived, non-native residues attached to its terminus. ...
www.neb.com/nebecomm/tech_reference/protein_expres...
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PLoS ONE: Inhibition of Anthrax Lethal Toxin-Induced Cytolysis of ...
Furthermore, celastrol blocked stimulation of IL-18 processing, .... GST or GST- S5a coupled to sepharose (20 µL of 0.5 mg protein/mL resin) was combined with 50 µg ..... by entry of its...
www.plosone.org/article/info:doi%2F10.1371%2Fjourn...
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Drosophila melanogaster
Identification and Functional Characterization of N- Terminally Acetylated Proteins in ... the second step, the acetylation of the amino-terminus is catalyzed ...
assets0.pubget.com/pdf/19885390.pdf
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Heat shock causes a decrease in polysomes and the appearance of
Key words: Heat shock, Trypanosoma brucei, Stress granules, eIF2 alpha, P-bodies ... All Versions of this Article: jcs.031823v1; 121/18/3002 most recent
jcs.biologists.org/cgi/content/full/121/18/3002
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Export Service Bulletin
Remover Set (T/N 070AZ-S5A-011). Insert the terminal remover into the center row cavity of the ... 18. If the original 16P connector is not heat damaged ...
www.hondalac.com/service/Bulletins/x04-015e.pdf
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Molecular Clustering of STIM1 with Orai1/CRACM1 at the Plasma ...
... N terminus of enhanced (e)GFP (PH-eGFP; Várnai and Balla, 1998) was obtained ... C, and Supplemental Figure S5A) and substantially increased molecular ...
www.molbiolcell.org/cgi/content/full/20/1/389
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Oxygen-Dependent Ubiquitination and Degradation of Hypoxia ...
A Flag epitope tag (DYKDDDDK) was added to the N terminus of the VHL cDNA open reading frame. ... The S5a column binds with high affinity to polyubiquitinated ...
mcb.asm.org/cgi/content/full/22/15/5319
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The Regulatory Particle of the Saccharomyces cerevisiae Proteasome --
We therefore chose to express recombinant forms of these genes from a single plasmid containing the RPT1 promoter and a His6 epitope at the N terminus of the gene to be expressed.
mcb.asm.org/cgi/content/full/18/6/3149
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Supporting Material
(A) N-terminal GFP-fusions of PIP5K1A (lane 1), S5a (lane 2) and full-length human (lane 3) ... Bioinformatics 18: 333-334. Nei, M., and Gojobori, T. 1986. ...
171.66.122.45/content/suppl/2007/08/02/gr.6252107....
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